| 胶束电动毛细管色谱分离氨基酸和磷酸化氨基酸 |
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| 引用本文： | 刘笔锋,曾永辉,吕应堂. 胶束电动毛细管色谱分离氨基酸和磷酸化氨基酸[J]. 分析科学学报, 2001, 17(2): 102-105 |
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| 作者姓名： | 刘笔锋 曾永辉 吕应堂 |
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| 作者单位： | 武汉大学植物发育生物学教育部重点实验室,武汉大学植物发育生物学教育部重点实验室,武汉大学植物发育生物学教育部重点实验室 武汉,430072,武汉,430072,武汉,430072 |
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| 基金项目： | 国家自然科学基金 (No.3 9780 0 1 5 ),教育部博士点基金和骨干教师培养计划资助 |
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| 摘 要： | 本文报道了胶束电动毛细管色谱分离、汞灯诱导荧光电荷耦合器件检测分析氨基酸和磷酸化氨基酸的 4-氟 - 7-硝基苯 - 2 - 口恶 - 1 ,3-丫唑衍生物。研究表明 ,在 p H9.35的 1 0 mmol/L硼砂和 1 0 mmol/L十二烷基硫酸钠的电泳缓冲介质中 ,5种氨基酸和 3种磷酸化氨基酸在 1 0 min内完全分离 ,检测灵敏度为 1 .2 1× 1 0 - 8～ 5 .2 1×1 0 - 8mol/L ,分离效率达 7.3× 1 0 5～ 3.0× 1 0 5/m理论塔板数 ,结果令人满意
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| 关 键 词： | 胶束电动毛细管色谱 氨基酸 磷酸化氨基酸 荧光检测 |
| 文章编号： | 1006-6144(2001)02-0102-04 |
| 修稿时间： | 2000-09-13 |
Separation of Amino Acids and Phosphoamino Acids as Their Derivatives by Micellar Electrokinetic Capillary Chromatography |
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| LIU Bi-feng,ENG Yong-hui,LU Ying-tang. Separation of Amino Acids and Phosphoamino Acids as Their Derivatives by Micellar Electrokinetic Capillary Chromatography[J]. Journal of Analytical Science, 2001, 17(2): 102-105 |
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| Authors: | LIU Bi-feng ENG Yong-hui LU Ying-tang |
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| Affiliation: | Key Laboratory of MOE for Plant Developmental Biology, Wuhan University,;Key Laboratory of MOE for Plant Developmental Biology, Wuhan University,;Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, |
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| Abstract: | The separation of amino acids and phosphoamino acids as their 4-fluoro-7-nitroben-2-oxa-1,3-diazple fluorescent derivatives was achieved by micellar electrokinetic capillary chromatography with lamp induced fluorescence-cooled charged coupled device detection. Some parameters such as precapillary derivatization conditions and separation conditions were discussed and optimized. With a buffer of pH 9.35 10 mmol/L borate containing 10 mmol/L SDS, the 5 amino acids and 3 phosphoamino acids were separated in 10 min. The detection limits were 1.21×10-8～5.21×10-8 mol/L with separation efficiency of 7.3×105～3.0×105/m. |
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| Keywords: | Micellar electrokinetic capillary chromatography Amino acids Phosphoamino acids Fluorescence detection |
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