不同生境条件下藻蓝蛋白活体荧光光谱特性研究

蓝藻生物量检测技术发展是应对目前频繁发生的水华事件的重要环节。藻蓝蛋白作为蓝藻的特异性蛋白，在一定程度上比叶绿素更能准确反应自然水体中的蓝藻生物量，因而成为蓝藻生物量检测技术的重要指标。本文利用三维荧光光谱技术，以不同光照、不同生长期的铜绿微囊藻、鱼腥藻活体为研究对象，比较了单点法和包络法两种光谱解析方法的可靠性，探究了不同生境条件下的藻蓝蛋白活体荧光光谱特性。结果表明：(1)荧光光谱强度随生长期延长而增大；(2)采用包络法解析藻蓝蛋白特征荧光光谱的方法比单点法更为可靠；(3)在不同生境条件下，铜绿微囊藻藻蓝蛋白活体荧光激发波长基本保持614 nm、发射波长基本保持654 nm不变，鱼腥藻藻蓝蛋白活体荧光激发波长随生长期在610和620 nm之间波动减小，发射波长随生长期在650和660 nm之间波动增大。这种波动与藻种样品颗粒度大小和光谱扫描模式有关。该研究结果为发展蓝藻生物量活体荧光监测技术发展提供了实验基础。

Research on Fluorescence Spectral Characteristics of Phycocyanin under Different Habitat Conditions

The development of cyanobacterial biomass detect ion technology is an important part to deal with the frequent occurrence of water bloom events.Phycocyanin,to some extent,as a special protein of cyanoba cteria,is more accurate reaction in natural water cyanobacterial biomassthan ch lorophyll,thus become an important index in cyanobacterial biomass detection.T his paper,with different light intensity and different growth stages as the res earch objects.compares the reliability of the two methods of the envelope metho d and the single point method,exploring the fluorescence spectral characterist ics of phycocyanin in vivo Microcystis Anabaena by using three-dimensional fluo rescence spectroscopy.The results show that:(1) The fluorescence intensity inc reases with the growth of long-term;(2) It is more reliable to use envelope me thod to analyze the characteristic fluorescence spectra of the fluorescent spect rum than the single point method.(3) The fluorescence spectra of Phycocyanin in vivo Microcystis were basically unchanged in EX=614 nm/ EM=654 nm.The excitati on wavelength of the phycocyanin fluorescence in vivo Microcystis decreases with the growth with 610 and 620 nm in the long term,and the emission wavelength va ried between 650 and 660 nm,which is related to the sample particle size and sp ectral scanning mode.This study provided the experimental basis for the develop ment of the fluorescent detection technology of cyanobacteria.