| 薄层色谱-荧光法测定黄连生物碱分配系数 |
| |
| 引用本文： | 贺凯,李学刚,陈红英,叶小利,邓亚飞,陈新,孙胜亮.薄层色谱-荧光法测定黄连生物碱分配系数[J].分析化学,2011,39(4):572-575. |
| |
| 作者姓名： | 贺凯 李学刚 陈红英 叶小利 邓亚飞 陈新 孙胜亮 |
| |
| 作者单位： | 西南大学生命科学学院;西南大学药学院药用资源化学研究所; |
| |
| 基金项目： | 国家科技部“重大新药创制”专项项目(No.2010ZX09401-306-3-10); 重庆市十一五重大新药创制项目(No.2008AA5021); 重庆科委攻关项目(No.CSTC2010AC5007); 中央高校基本科研业务费专项(No.XDJK2009C095)资助 |
| |
| 摘 要： | 建立了薄层色谱和荧光分析法测定黄连生物碱在高速逆流色谱溶剂体系中分配系数的方法.样品在溶剂体系中分配平衡后,取等量的上下相,经点板、展开、刮板,提取后用荧光分光光度法检测样品的荧光值(激发波长365 nm,发射波长409 nm),并计算样品在溶剂体系中的分配系数.在一定的浓度范围内,4种生物碱的含量与荧光强度呈良好的线...
|
| 关 键 词： | 薄层色谱 荧光分析 高速逆流色谱 分配系数 溶剂体系 |
Determination of Partition Coefficient of Alkaloids from Coptis Chinensis Franch by Thin Layer Chromatograph-Fluorometric Spectrophotometry |
| |
| HE Kai,LI Xue-Gang,CHEN Hong-Ying,YE Xiao-Li,DENG Ya-Fei,CHEN Xin,SUN Sheng-Liang.Determination of Partition Coefficient of Alkaloids from Coptis Chinensis Franch by Thin Layer Chromatograph-Fluorometric Spectrophotometry[J].Chinese Journal of Analytical Chemistry,2011,39(4):572-575. |
| |
| Authors: | HE Kai LI Xue-Gang CHEN Hong-Ying YE Xiao-Li DENG Ya-Fei CHEN Xin SUN Sheng-Liang |
| |
| Institution: | HE Kai1,LI Xue-Gang2,CHEN Hong-Ying2,YE Xiao-Li1,DENG Ya-Fei2,CHEN Xin2,SUN Sheng-Liang1 1(School of Life Science,Southwest University,Chongqing 400715)2(Chemistry Institute of Pharmaceutical Resources,College of Pharmaceutical Sciences,Chongqing 400716) |
| |
| Abstract: | A thin layer chromatograph(TLC) coupled with fluorometric spectrophotometric method has been established to determine the partition coefficient of alkaloids extracted from Coptis chinensis Franch in solvent systems of high speed counter-current chromatography(HSCCC).After samples was equilibrated in solvent system,equal volume of upper and lower phase was spreaded with TLC method respectively and then extracted to measure the fluorescence intensity with fluorometric spectrophotometry(Lex 365 nm,Lem 409 nm) ... |
| |
| Keywords: | Thin layer chromatography Fluorometric method High speed counter-current chromatography Partition coefficient Solvent system |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
