| 基于丝网印刷碳电极的微囊藻毒素-(亮氨酸-精氨酸)的电流型免疫传感器 |
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| 引用本文： | 陈向强,何苗,施汉昌,蔡强.基于丝网印刷碳电极的微囊藻毒素-(亮氨酸-精氨酸)的电流型免疫传感器[J].分析化学,2011,39(4):443-448. |
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| 作者姓名： | 陈向强 何苗 施汉昌 蔡强 |
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| 作者单位： | 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室;浙江清华长三角研究院生态环境研究所; |
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| 基金项目： | 国家高新技术发展计划(Nos.2007AA06Z419,2009AA06A417)资助项目 |
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| 摘 要： | 将微囊藻毒素-(亮氨酸-精氨酸)-鸡卵白蛋白 (Microcystin-(leucinc-arginine)-ovalbumin,MCLR-OVA)固定在锇联吡啶聚(4-乙烯基吡啶)聚合物修饰的丝网印刷碳电极表面,制备了一种检测 MCLR的电流型免疫传感器.该传感器基于间接竞争免疫分析模式,以辣根过氧化物酶偶联的羊抗鼠...
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| 关 键 词： | 免疫传感器 微囊藻毒素-(精氨酸-亮氨酸) 锇氧化还原聚合物 丝网印刷碳电极 |
An Amperometric Immunosensor for Microcystin-(Leucine-Arginine) Based on Screen-Printed Carbon Electrode |
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| CHEN Xiang-Qiang,HE Miao,SHI Han-Chang,CAI Qiang.An Amperometric Immunosensor for Microcystin-(Leucine-Arginine) Based on Screen-Printed Carbon Electrode[J].Chinese Journal of Analytical Chemistry,2011,39(4):443-448. |
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| Authors: | CHEN Xiang-Qiang HE Miao SHI Han-Chang CAI Qiang |
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| Institution: | CHEN Xiang-Qiang1,HE Miao1,SHI Han-Chang1,CAI Qiang1,2 1(State Key Joint Laboratory of Environment Simulation and Pollution Control,Department of EnvironmentalScience and Engineering,Tsinghua University,Beijing 100084)2(Institute of Ecology and Environment,Yangtze Delta Region Research Institute ofTsinghua University,Jiaxing 314050) |
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| Abstract: | An amperometric immunosensor for the detection of microcystin-(leucine-arginine)(MCLR) was prepared by immobilizing an MCLR-ovalbumin(MCLR-OVA) conjugate to an osmium bipyridyl complex-attached poly(4-vinylpyridine) membrane modified onto a screen-printed carbon electrode.The immunosensor was based on the indirect competitive immunoassay format with the labeled agent of horseradish peroxidase-conjugated goat anti-mouse immunoglobulin-G antibody.The current response decreased proportionally with the concentr... |
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| Keywords: | Immunosensor Microcystin-(leucine-arginine) Osmium redox polymer Screen-printed carbon electrode |
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