Far-from-equilibrium fluctuations triggering RNA de novo synthesis |
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| Authors: | A Fernández |
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| Institution: | (1) Frick Laboratory, Princeton University, Princeton, USA;(2) Present address: Max-Planck Institut für Biophysikalische Chemie, Göttingen, F.R.G. |
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| Abstract: | The fluctuations for very low total template concentration (5–104 molecules/cm3) are calculated theoretically for a self-replicating RNA system catalyzed by highly purifiedQ replicase, by means of a center manifold stochastic treatment. The results provide an explanation of the experimental observation that at 0.15 mM nucleoside triphosphate concentration, no RNA can be synthesizedde novo, although the templatedirected synthesis proceeds normally. The role of intrinsic template fluctuations, under far-from-equilibrium conditions; in triggeringde novo (template-free) replication is elucidated. |
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| Keywords: | RNA polymerization-elongation kinetics self-replicating RNA far-from equilibrium fluctuations center-manifold reduction of relaxing kinetic modes |
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