常见野生蘑菇的红外光谱及其二维相关红外光谱的鉴别

采用傅里叶变换红外光谱结合相关性分析、二阶导数谱和二维相关谱对13种常见野生蘑菇进行鉴别研究。结果显示13种野生蘑菇的红外光谱光谱特征相似，主要由蛋白质、多糖的吸收组成，13种样品的相关系数最小为0.779，最大值为0.960。在1 700~1 400和1 400~800 cm-1范围的二阶导数光谱中，各样品吸收峰的强度、位置和形状均有明显差异。二维相关红外光谱在1 380~1 680 cm-1范围的强自动峰整体相近，但自动峰和交叉峰位置、形状存在差异；在920~1 230 cm-1的范围，各样品的自动峰和交叉峰的数量、强度和位置差异明显。结果表明傅里叶变换红外光谱结合相关性分析、二阶导数谱和二维相关谱有望发展为区分不同种类蘑菇的快速方法。

Discrimination of Common Wild Mushrooms by FTIR and Two-Dimensional Correlation Infrared Spectroscopy

Fourier Transform Infrared Spectroscopy combined with correlation analysis, second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy was used to discriminate 13 species of wild mushrooms. The results showed that the general characteristics of absorption bands in the original spectra were similar, which were mainly composed of protein and polysaccharides. Correlation analysis was applied for the spectra of samples, and the correlation coefficient between Termitomyces eurrhizus and Tylopilus plumbeoviolaceoides was 0.779, which was the minimum, while the correlation coefficient between Pleurotus lignatilis Gill and Hygrophorus lucorum Kalchbr was 0.960, which was the maximum. Coprinus comatus had little correlation with other samples. The differences of intensity, position and shape were observed in second derivative spectra in the range of 1 700～1 400 and 1 400～800 cm-1. In two-dimensional correlation spectra, the differences of the position or form of auto-peaks and cross peaks have been found in the range of 1 380~1 680 cm-1. The number, position and intensity of auto-peaks and cross peaks were obviously different in the range of 920~1 230 cm-1. The study demonstrates that Fourier Transform Infrared spectroscopy combined with correlation analysis, second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy might be developed as a rapid method to discriminate different kinds of mushrooms.