| 高速逆流色谱与硅胶柱色谱结合分离制备高纯度芦荟素异构体 |
| |
| 引用本文： | 黄丹凤,曹学丽,赵华,董银卯. 高速逆流色谱与硅胶柱色谱结合分离制备高纯度芦荟素异构体[J]. 色谱, 2006, 24(1): 42-45 |
| |
| 作者姓名： | 黄丹凤 曹学丽 赵华 董银卯 |
| |
| 作者单位： | Beijing Key Laboratory of Plant Resources Research and Development, School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100037, China |
| |
| 基金项目： | 北京市教委科技发展计划项目;北京市优秀人才培养基金 |
| |
| 摘 要： | 芦荟素是芦荟叶中主要的蒽醌类成分,通常以芦荟素A和B两种非对映异构体形式并存,目前已成为许多芦荟产品质量控制指标之一。采用高速逆流色谱和硅胶柱色谱结合的方法,从老芦荟干粉中分离制备了高纯度的芦荟素A(纯度为98%)和芦荟素B(纯度为96%)样品,并采用快原子轰击质谱(FAB-MS)和核磁共振氢谱(1H NMR)以及GOESY(gradient-enhanced nuclear Overhauser effect spectroscopy)等方法对所得的两个芦荟素异构体的立体构象进行了确认。该法具有制备量大和分离效率高的特点。
|
| 关 键 词： | 分离制备 高速逆流色谱 高效液相色谱 硅胶柱色谱 芦荟素异构体 |
| 文章编号： | 1000-8713（2006）01-0042-04 |
| 收稿时间： | 2005-01-17 |
| 修稿时间： | 2005-01-17 |
Preparative Separation of Aloin Diastereoisomers by High-Speed Countercurrent Chromatography Combined with Silica Gel Column Chromatography |
| |
| HUANG Danfeng,CAO Xueli,ZHAO Hua,DONG Yinmao. Preparative Separation of Aloin Diastereoisomers by High-Speed Countercurrent Chromatography Combined with Silica Gel Column Chromatography[J]. Chinese journal of chromatography, 2006, 24(1): 42-45 |
| |
| Authors: | HUANG Danfeng CAO Xueli ZHAO Hua DONG Yinmao |
| |
| Affiliation: | Beijing Key Laboratory of Plant Resources Research and Development, School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100037, China |
| |
| Abstract: | Aloin,naturally a mixture of two diastereoisomers,aloin A and aloin B,is the major(anthraquinone) in aloe,and now served as one of the important control constituents in most of the commercial aloe products.High-speed countercurrent chromatography(HSCCC) combined with(silica) gel column chromatography was developed for the preparative separation of the two individual(aloins).Aloin A(98%) and aloin B(96%) were obtained.Fast atom bombardment mass spectrometry(FAB-MS),()~1H nuclear magnetic resonance(()~1H NMR) and GOESY(gradient-enhanced nuclear Overhauser effect spectroscopy) were employed for the elucidation of their structure conformation.The developed method is of high preparative capacity and high efficiency in resolution. |
| |
| Keywords: | high-speed countercurrent chromatography(HSCCC) silica gel column chromatography preparative separation high performance liquid chromatography(HPLC) aloin diastereoisomers |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《色谱》浏览原始摘要信息 |
|
点击此处可从《色谱》下载全文 |
