Carotenoid incorporation into microsomes: yields, stability and membrane dynamics |
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Authors: | Socaciu C Jessel R Diehl H A |
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Institution: | Department of Chemistry and Biochemistry, University of Agricultural Sciences and Vetinary Medicine, Romania. |
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Abstract: | The carotenoids beta-carotene (BC), lycopene (LYC), lutein (LUT), zeaxanthin (ZEA), canthaxanthin (CTX) and astaxanthin (ASTA) have been incorporated into pig liver microsomes. Effective incorporation concentrations in the range of about 1-6 nmol/mg microsomal protein were obtained. A stability test at room temperature revealed that after 3 h BC and LYC had decayed totally whereas, gradually, CTX (46%), LUT (21%), ASTA (17%) and ZEA (5%) decayed. Biophysical parameters of the microsomal membrane were changed hardly by the incorporation of carotenoids. A small rigidification may occur. Membrane anisotropy seems to offer only a small tolerance for incorporation of carotenoids and seems to limit the achievable incorporation concentrations of the carotenoids into microsomes. Microsomes instead of liposomes should be preferred as a membrane model to study mutual effects of carotenoids and membrane dynamics. |
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Keywords: | Carotenoids Cholesterol Liver microsomes Incorporation Membrane fluidity Anisotropy |
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