UV LASER INDUCED RNA-PROTEIN CROSSLINKS AND RNA CHAIN BREAKS IN TOBACCO MOSAIC VIRUS RNA in situ |
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| Authors: | E N DOBROV Z KH ARBIEVA E K TIMOFEEVA R O ESENALIEV A A ORAEVSKY D N NIKOGOSYAN |
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| Institution: | A.N. Belozersky Laboratory of Molecular Biology;Department of Virology of Moscow State University, Moscow 119899;Institute of Spectroscopy, USSR Academy of Sciences, Troitzk, Moscow Region 142092, USSR |
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| Abstract: | The efficiency of RNA-protein crosslink and RNA chain break formation under nanosecond or picosecond UV-laser pulse irradiation of tobacco mosaic virus was determined. It was found that on high-intensity UV-laser irradiation the quantum yields of both reactions increase considerably as compared to the usual (low-intensity) UV-irradiation. The RNA-protein crosslink quantum yield was found to be 1.8 x 10(-5) and 1.2 x 10(-4) and that of RNA chain breaks 1.7 x 10(-4) and 8.9 x 10(-4) for nanosecond and picosecond irradiation, respectively. |
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