牛血清蛋白对手性物质的毛细管电泳拆分

采用聚氧乙烯涂层毛细管柱，以牛血清蛋白(BSA)为手性添加剂，运用毛细管电泳电导检测对手性药物沙丁胺醇和多沙唑嗪以及DL-组氨酸、色氨酸、苏氨酸进行拆分。考察了涂层柱的稳定性和重现性。对影响手性分离的主要因素：缓冲溶液的浓度和酸度、BSA的浓度、有机溶剂的种类和浓度以及分离电压进行了系统的研究。结果表明：在选择的最佳实验条件下，各对映异构体在22min内得到基线分离。

The Separation of the Chiral Drug and Amino Acid by Capillary Electrophoresis with Conductance Detection Based on Bovine Serum Albumin as Enantioselective Protein

Using polyglycol coated capillary column, the chiral drugs salbutamol and doxazosin, DL-histidine, DL-tryptophan, DL-threonine were separated into two enantiomers by capillary electrophoresis with conductance detection, when bovine serum albumin (BSA) was used as a chiral resolving agent. The stability and repeatability of coated capillary column were tested. The main factors which affect the chiral separation, concentration and pH of the background electrolyte, concentration of bovine serum albumin, the kinds and concentration of organic solvents, separation voltage on the enantiomeric separation were investigated in detail. It was found that baseline separation of the enantiomers could be accomplished in 22 min under the optimum condition.