| 盐酸鸟嘌呤与牛血清白蛋白的相互作用 |
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| 引用本文： | 李建晴,蔡雪梅,董川. 盐酸鸟嘌呤与牛血清白蛋白的相互作用[J]. 理化检验(化学分册), 2011, 0(3) |
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| 作者姓名： | 李建晴 蔡雪梅 董川 |
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| 作者单位： | 晋中学院化学化工学院;山西大学环境科学与工程研究中心; |
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| 基金项目： | 国家自然科学基金(20875059) |
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| 摘 要： | 应用荧光光谱、紫外-可见分光光度法研究了盐酸鸟嘌呤(GH)与牛血清白蛋白(BSA)的相互作用。结果表明:GH能猝灭BSA的荧光强度,其猝灭机理为静态猝灭。采用位点结合模型公式和热力学公式计算了结合常数、结合位点数及结合类型。用同步荧光技术研究GH对BSA构象的影响。
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| 关 键 词： | 盐酸鸟嘌呤 牛血清白蛋白 荧光光谱 荧光猝灭 |
Interaction Between Guanine Hydrochloride and Bovine Serum Albumin |
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| LI Jian-qing,CAI Xue-mei,DONG Chuan. Interaction Between Guanine Hydrochloride and Bovine Serum Albumin[J]. Physical Testing and Chemical Analysis Part B:Chemical Analgsis, 2011, 0(3) |
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| Authors: | LI Jian-qing CAI Xue-mei DONG Chuan |
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| Affiliation: | LI Jian-qing~1,CAI Xue-mei~1,DONG Chuan~2 (1.Dept.of Chem.and Chem.Eng.,Jinzhong College,Jinzhong 030600,China,2.Research Center of Environmental Science and Engineering,Shanxi University,Taiyuan 030006,China) |
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| Abstract: | Mechanism of the interaction between guanine hydrochloride(GH) and bovine serum albumin (BSA) was studied by fluorospectrometry and UV-VIS spectrophotometry.Quenching of fluorescence of BSA was observed due to its reaction with GH,and the interaction was found to he a static quenching process.The binding constants,number of binding sites and types of binding force were found by applying the equation of site-binding model and thermodynamic equations.Synchronous fluorescence spectrometry was used in the study... |
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| Keywords: | Guanine hydrochloride Bovine serum albumin Fluorescence spectroscopy Fluorescence quenching |
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