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| 激光激发荧光谱的双光路多道分析方法研究 | |
| 引用本文： | 左浩毅,程娟,贺应红,杨经国. 激光激发荧光谱的双光路多道分析方法研究[J]. 光谱学与光谱分析, 2004, 24(4): 396-398 |
| 作者姓名： | 左浩毅 程娟 贺应红 杨经国 |
| 作者单位： | 四川大学物理系,四川,成都,610064;四川大学物理系,四川,成都,610064;四川大学物理系,四川,成都,610064;四川大学物理系,四川,成都,610064 |
| 基金项目： | 国家自然科学基金(60078020)资助项目 |
| 摘 要： | 在脉冲激光激发荧光的研究中由于入射激光强度的起伏会导致所激发荧光的强度随之波动 ,从而给荧光光谱及其待测物质测量结果带来较大误差。采用双光路法 (即在采集荧光谱的同时也采集激发激光强度 ,利用荧光强度与激发光强度的线性相关 ,对实验数据进行相关分析 )可以有效地克服激发光波动对荧光光谱的影响 ,显著降低了测量误差。用此法对若丹明 6G和十二烷基硫酸纳 (SDS)对R6G的荧光增强进行测量 ,可得到相关系数大于 0 9的测量结果。在激发光波动较大时双光路法与单光路采样平均法相比有明显优越性。 |
| 关 键 词： | 激光激发荧光 双光路 相关分析 |
| 文章编号： | 1000-0593(2004)04-0396-03 |
| 修稿时间： | 2002-09-28 |
Double Light Beam Multi-Channel Analysis for Laser Induced Fluorescence Spectroscopy |
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| Hao-yi Zuo,Juan Cheng,Ying-hong He,Jing-guo Yang. Double Light Beam Multi-Channel Analysis for Laser Induced Fluorescence Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2004, 24(4): 396-398 | |
| Authors: | Hao-yi Zuo Juan Cheng Ying-hong He Jing-guo Yang |
| Affiliation: | Department of Physics, Sichuan University, Chengdu 610064, China. |
| Abstract: | The incident pulse intensity wave will cause the induced fluorescence wave, which will lead to the obvious error of experiment results. A double-light-beam correlation method between the incident laser and the fluorescence was used to decrease such error efficiently in the experiments. A good correlation factor over 0.9 was obtained by the method in the experiment of R6G fluorescence enhanced by SDS. The method is better than sampling-averaging method when the incident laser intensity has more waves. |
| Keywords: | Laser induced fluorescence Double light beam Correlation analysis |
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