Study of two-dimensional liquid chromatography with high temperature NPLC and room temperature RPLC

To overcome the peak band broadening and to increase the peak capacity and separation efficiency of a two-dimensional liquid chromatographic system, a high-temperature normal phase liquid chromatography (HTNPLC) was used as the first dimension (1^st-D), and a RPLC was used as the second dimension (2^nd-D). The sample was first separated on the 1^st-D CN column and the primary eluent stored in the sampling-loop system alternatively (in HTNPLC×RPLC mode) or selectively (in HTNPLC/RPLC mode) and was then transferred to 2^nd-D C₁₈ column for further separation. The resolution and separation efficiency of the systems were greatly improved. The systems were evaluated by analyzing several polycyclic aromatic hydrocarbons and Glycyrrhiza uralensis extract. __________ Translated from Journal of Instrumental Analysis, 2008, 27(1) (in Chinese)