Development and validation of a high-performance liquid chromatography method for quantification of egonol and homoegonol in Styrax species |
| |
| Authors: | Moraes Ana C G Bertanha Camila S Gimenez Valéria M M Groppo Milton Silva Márcio L A Cunha Wilson R Januário Ana H Pauletti Patrícia M |
| |
| Affiliation: | 1. Universidade de Franca, , 201, 14404‐600 Franca, S?o Paulo, Brazil;2. Centro Universitário Claretiano, , 466, 14.300‐000 Batatais, S?o Paulo, Brazil;3. Faculdade de Filosofia, Ciências e Letras, Universidade de S?o Paulo, , 3900, 14040‐901 Ribeir?o Preto, S?o Paulo, Brazil |
| |
| Abstract: | Styrax camporum Pohl, known in Brazil as 'estoraque do campo' or 'cuia de brejo', has been used in the treatment of gastrointestinal diseases. The therapeutic action of S. camporum has been attributed to the ethyl acetate fraction, although the chemical composition of this fraction has not yet been analyzed. In this study, a high-performance liquid chromatography photodiode array detection (HPLC-PAD) method for analysis of Brazilian Styrax species has been developed. The compounds egonol (1) and homoegonol (2) were found to be present in all the samples investigated by HPLC. These compounds were isolated by open column chromatography followed by preparative TLC, and were identified by 1H NMR. Compounds 1 and 2 were thus proposed as phytochemical markers for Styrax, owing to their biological properties and presence in other Styrax species. The developed method has been validated and successfully applied for quantification of 1 and 2 in S. camporum dried leaves and crude ethanolic extracts from S. ferrugineus and S. pohlii aerial parts. |
| |
| Keywords: | HPLC‐PAD quantitative determination egonol homoegonol Styrax camporum |
| 本文献已被 PubMed 等数据库收录！ |
|
