A lysosomal targeting fluorescent probe and its zinc imaging in SH-SY5Y human neuroblastoma cells |
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| Authors: | Houna Duan Yu Ding Chusen Huang Weiping Zhu Rui Wang Yufang Xu |
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| Institution: | 1. State Key Laboratory of Bioreactor Engineerring, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;2. The Education Ministry Key Laboratory of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;3. Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China |
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| Abstract: | Zinc plays a significant role in oxidative balance and central nervous systems. Herein, we reported a highly sensitive fluorescent probe DR, bearing a morpholine group and a BPEN ligand in the naphthalimide fluorophore. Upon Zn2+ binding, DR exhibited remarkable fluorescence enhancement, and showed high sensitivity to Zn2+ with the association constant of 4.9 × 108 L/mol, and the detection limit of 15 nmol/L. Confocal imaging experiments indicated that DR was able to localize to lysosomes in MCF-7 cells. Moreover, upon H2O2 stimulation in SH-SY5Y cells, endogenous release of Zn2+ was observed. |
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| Keywords: | Naphthalimide Zinc ions Cellular imaging Lysosome Fluorescent probe |
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