A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation |
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| Authors: | Song Li Xupeng Cao Yan Wang Zhen Zhu Haowei Zhang Song Xue Jing Tian |
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| Affiliation: | 1.School of Bioengineering,Dalian Polytechnic University,Dalian,China;2.Marine Bioengineering Group,Dalian Institute of Chemical Physics, CAS,Dalian,China;3.Novel Technology and Materials for Separation and Detection of Biomolecules Group,Dalian Institute of Chemical Physics, CAS,Dalian,China |
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| Abstract: | ![]()
With the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described to meet the characteristics of microalgae cells and eliminate the error caused by over-alkylation. Using Chlamydomonas reinhardtii as the model, the prepared sample was tested by standard LC-MS/MS and compared with the previous reports. The results showed mFASP is suitable for most of occasions of microalgae proteomics studies. |
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