The use of supercritical fluid extraction for the determination of 4-deoxynivalenol in grains: The effect of the sample clean-up and analytical methods on quantitative results |
| |
Authors: | E P Järvenpää S L Taylor J W King R Huopalahti |
| |
Institution: | (1) Laboratory of Food Chemistry, University of Turku, Department of Biochemistry and Food Chemistry, 20500 Turku, Finland;(2) Food Quality & Safety Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service/USDA, 61604 Peoria, IL, USA |
| |
Abstract: | Summary Deoxynivalenol (DON) is one of the trichothecene mycotoxins produced byFusarium molds in grains. Polar cosolvents in supercritical carbon dioxide (SC-CO2) are needed to extract and isolate the polar DON moiety. This unfortunately results in the extraction of many interfering
compounds from the grains into the extracts obtained by supercritical fluid extraction (SFE). Analysis of DON by high performance
liquid chromatography (HPLC) using ultraviolet detection (UV) does not provide a specific detection method, although specific
detection of DON can be enhanced by using purification steps after SFE. Alternatively, combining SFE with an immunoaffinity
method can improve detection specificity and sample cleanup. In this study, SFE was employed to determine DON in grains and
cereal products. The effectiveness of the SFE method was compared with two different solvent extraction methods. The extracted
DON was quantitatively determined by HPLC-UV using external standardization or competitive enzymelinked immunosorbent assay
(ELISA). In some cases, extracts were purified prior to quantitative analysis of the DON by using solvent partitioning, and/or
solid phase extraction, or immunoaffinity columns. Therefore, this paper describes the analysis of DON in cereals using different
extraction, cleanup and analysis methods.
Names are necessary to report factually on available data: however, the USDA neither guarantees nor warrants the standard
of the product, and the use of the name by USDA implies no approval of the products to the exclusion of others that may also
be suitable. |
| |
Keywords: | Supercritical fluid extraction Column liquid chromatography Cleanup Deoxynivalenol in cereals Immunoassay |
本文献已被 SpringerLink 等数据库收录! |
|