Linear Dichroism of Chloroplasts and Subchloroplast Fractions Oriented by Flow |
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| Authors: | F. Tjerneld B. Norden H. E. Akerlund B. Andersson P. -A. Albertsson |
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| Affiliation: | Departments of Biochemistry 1 and Inorganic Chemistry 1 , University of Lund, Chemical Center , P.B. 740, S-220 07, Lund, Sweden |
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| Abstract: | ![]()
Abstract Chloroplasts as well as subchloroplast vesicles show linear dichroism (LD) when subjected to a hydrodynamic gradient. LD is positive for the ππ? in-plane polarized bands indicating that the chlorophyll porphyrin ring is in average oriented parallel1 to the elongated membrane surfaces. The vesicle fraction enriched inphotosystem I1 shows lower LD/A at 660 nm indicating lower average orientation of chlorophyll and the fraction enriched in photosystem I shows higher LD/A at 700 nm indicating that P700 is more oriented than the bulk chlorophyll. |
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