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Heteronuclear Multiple-Quantum Coherence NMR Spectroscopy of Im-cyt C |
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| Authors: | Weiping Shao Xiaoling Huang Gaohua Liu Houming Wu Wenxia Tang |
| Affiliation: | 1. State Key Lab. of Coord. Chemistry , Nanjin University , Nanjin, 210093, China;2. State Key Lab. of Bioorganic &3. Natural Products Chemistry , Shanghai Institute of Organic Chemistry , Shanghai, 200032, China |
| Abstract: | Some 13C chemical shifts of the CHn groups in the aliphatic side chains of Im-cyt c have been determined for the first time based on the H chemical shifts of their attached protons with the aid of heteronuclear multiple-quantum coherence (HMQC) spectroscopy. Comparison of chemical shifts of these specifically assigned 13C and H resonances from Im-cyt c with those from cyt c indicates that 13C-NMR spectra may provide an opportunity to probe the electronic structure and conformational changes induced by axial ligand substitution. |
| Keywords: | Im-cyt c HMQC 13C resonance assignments |