Cu-Chelex as a selective adsorbent for o-nitroso derivatives of phenolic substances |
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Authors: | Takafumi Ohta Kohji Shikinami Yukiyo Iga and Shoji Takitani |
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Institution: | (1) Faculty of Pharmaceutical Sciences, Science University of Tokyo, 12 Ichigaya-Funagawara-Machi, Shinjuku-Ku, 162 Tokyo, Japan |
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Abstract: | Summary Chelex resins in several metal forms were tested for selective uptake of o-nitrosonaphthols and o-nitrosophenols from aqueous solution. Cu-Chelex was the most efficient resin over a wide pH range, and the taken-up nitroso compounds were effectively eluted with concentrated ammonia: acetonitrile (4:1, v/v) after washing the resin with acetonitrile to eliminate non-specific binding. The upper and lower limits of uptake capacity of the resin for 1-nitroso-2-naphthol were 0.5 mol/100 mg and 1.25 mol/100 mg, respectively. A preliminary recovery experiment using urine and cucumber juice showed that Cu-Chelex would be useful for screening of o-nitroso derivatives of phenolic substances in complicated samples such as biological fluids and foods. |
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