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| 血清总磷脂脂肪酸组分的固相萃取-气相色谱法分析 | |
| 引用本文： | 谭力,鞠熀先,黎介寿,周敏,李小双,柳晓泉.血清总磷脂脂肪酸组分的固相萃取-气相色谱法分析[J].分析科学学报,2006,22(2):125-128. |
| 作者姓名： | 谭力 鞠熀先 黎介寿 周敏 李小双 柳晓泉 |
| 作者单位： | 1. 南京大学化学系,生命分析化学教育部重点实验室,南京,210093;南京军区总医院普通外科研究所,南京,210002 2. 南京大学化学系,生命分析化学教育部重点实验室,南京,210093 3. 南京军区总医院普通外科研究所,南京,210002 4. 中国药科大学药代研究中心,南京,210009 |
| 基金项目： | 国家重点基础研究发展计划(973计划);国家重点基础研究发展计划(973计划) |
| 摘 要： | 以硅胶基质为固定相,建立了固相萃取-气相色谱(SPE-GC)分离检测血清总磷脂中脂肪酸含量的方法。优化了SPE的洗脱方式及甲醇洗脱量,探讨了衍生化步骤的优化条件。该法对磷脂中各脂肪酸组分测定的线性相关系数约为0.999,回收率在80%左右;日内误差小于4.8%,日间误差小于8.9%。用固相萃取柱分离磷脂简单、快速、有效,用气相色谱检测磷脂中脂肪酸组分方法稳定、可靠。 |
| 关 键 词： | 磷脂 脂肪酸 固相萃取柱 气相色谱 |
| 文章编号： | 1006-6144(2006)02-0125-04 |
| 收稿时间： | 2004-12-15 |
| 修稿时间： | 2005-03-11 |
Analysis of Fatty Acid of Phospholipids in Serum by Solid Phase Extraction-Gas Chromatography |
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| TAN Li,JU Huang-xian,LI Jie-shou,ZHOU Min,LI Xiao-shuang,LIU Xiao-quan.Analysis of Fatty Acid of Phospholipids in Serum by Solid Phase Extraction-Gas Chromatography[J].Journal of Analytical Science,2006,22(2):125-128. | |
| Authors: | TAN Li JU Huang-xian LI Jie-shou ZHOU Min LI Xiao-shuang LIU Xiao-quan |
| Institution: | 1. Key Laboratory of Analytical Chemistry for Life Science (Education Ministry of China |
| Abstract: | A analytical method for fatty acids profile of phospholipids in serum samples was developed by solid extraction column of silica gel-gas chromatography(SPE-GC).The model of elution and the amount of methanol used for solid phase extraction were optimized.A derivatization method without saponification step of the sample was proposed.The linearity of the measurement was good with the coefficients of more than 0.999.The recoveries of fatty acids were about 80%.Within-day and between-day RSD were less than(4.8%) and(8.9%,)respectively.The method for separation of phospholipids was simple,rapid and effective compared with thin-layer chromatography.Gas chromatographic detection was a stable and efficient method for the determination of fatty acids of phospholipids in serum samples. |
| Keywords: | Phospholipids Fatty acids Solid phase extraction Gas chromatography |
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