An investigation of the chemiluminescence reaction in the sodium hypochlorite–folic acid–emicarbazide hydrochloride system |
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Authors: | Feng Nie Yunhua He Jiuru Lu |
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Institution: | a Department of Chemistry, Hanzhong Teacher's College, Hanzhong, Shaanxi, 723000, PR China;b Department of Chemistry, Shaanxi Normal University, Xian 710062, PR China |
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Abstract: | A chemiluminescence method for the determination of folic acid by the sodium hypochlorite–folic acid–semicarbazide hydrochloride system with a new flow injection technique has been established. The new method can perform simple, sensitive and rapid determinations of folic acid. The response to the concentration of folic acid, in the range of 1.0×10−75.0×10−5 g/ml, is linear. The relative standard deviation of the method is 2.3% (Cs=4.0×10−6 g/ml, n=11). The detection limit is 2.7×10−8 g/ml. This method is suitable for automatic and continuous analysis, and has been successfully tested for the determination of folic acid in a folic acid tablet. |
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Keywords: | Chemiluminescence Flow injection Folic acid Sodium hypochlorite |
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