Evaluation of enzymatic kinetic parameters by thin-layer chromatography with radiometric detection

A radiochromatographic method is described for evaluating K_m and v_max based on a simple modification of the integrated Michaelis-Menten equation. Experimental points are ritted directly to this equation be an iterative technique, the validity of which is tested on simulated experimental data. Results for lysine, ornithine, and glutamic acid decarboxylases are reported.