用微束激光穿刺技术将商陆抗病毒蛋白(PAP)cDNA导入马铃薯的研究

以马铃薯(Solanum tuberosum L.)品种“津引8号”微薯切片作为外植体,采用微束激光穿刺技术获得了商陆抗病毒蛋白(PAP)cDNA的导入、整合与表达.实验首次利用庆大霉素筛选马铃薯再生苗,庆大霉素筛选浓度为80mg/L,共得到65株庆大霉素抗性苗,全部移栽成活.PCR扩增和PCR-Southern杂交分析表明,PAP cDNA已经稳定整合到马铃薯基因组.攻毒实验发现,多数转基因植株生长健康,对供试病毒PVX具有高度抗性.

INTRODUCTION OF PAP cDNA INTO POTATO BY THE LASER MICROBEAM PUNCTURE TECHNIQUES

The pokeweed antivirus protein (PAP) cDNA were successfully introduced into the microtuber discs of Potato (Solanum tuberosum L.) cultivars “Jinyin 8” by the laser microbeam puncture techniques (LMPT),which is the first report at home and abroad.65 regenerated plantlets,screened by gentamycin with the optimum concentration of 80mg/L,were obtained.The PAP cDNA was stably integrated into the genome of potato as assayed by PCR and PCR-Southern analyses.The test of PVX challenge showed that the transgenic plants had a significant higher resistance in comparison with the control.Most transgenic plants appeared phenotypically normal.