| 白藜芦醇浸提纯化过程在线检测条件的研究 |
| |
| 引用本文： | 沈会明,高丽娟,刘依然,姚腾豪,张丽华,王霞. 白藜芦醇浸提纯化过程在线检测条件的研究[J]. 光谱实验室, 2010, 27(5): 1821-1823 |
| |
| 作者姓名： | 沈会明 高丽娟 刘依然 姚腾豪 张丽华 王霞 |
| |
| 作者单位： | 辽宁科技大学化工学院,辽宁省鞍山市高新区千山中路185号,114051;辽宁省鞍山市第一中学,辽宁省鞍山市高新区鞍千路300号,114051 |
| |
| 基金项目： | 辽宁科技大学第五届大学生科研训练项目资助 |
| |
| 摘 要： | 通过实验确定了虎杖白藜芦醇乙醇浸提液的HPLC检测条件。最佳检测条件为,Extend反相ODS柱0.46cm×15cm,紫外检测器检测波长240nm,V(甲醇)∶V(水)=50∶50的流动相,1mL/min的洗脱流速,室温。该方法灵敏、方便、可靠、快速,适用于白藜芦醇提取、纯化中的在线检测。
|
| 关 键 词： | 虎杖 白藜芦醇 高效液相色谱 |
Study of on-Line Determination Conditions of Resveratrol During Extraction and Purification Resveratrol Process |
| |
| SHEN Hui-Ming,GAO Li-Juan,LIU Yi-Ran,YAO Teng-Hao,ZHANG Li-Hua,WANG Xia. Study of on-Line Determination Conditions of Resveratrol During Extraction and Purification Resveratrol Process[J]. Chinese Journal of Spectroscopy Laboratory, 2010, 27(5): 1821-1823 |
| |
| Authors: | SHEN Hui-Ming GAO Li-Juan LIU Yi-Ran YAO Teng-Hao ZHANG Li-Hua WANG Xia |
| |
| Affiliation: | SHEN Hui-Ming GAO Li-Juan LIU Yi-Rana YAO Teng-Hao ZHANG Li-Hua WANG Xia(School of Chemical Engineering,University of Science and Technology Liaoning,Anshan,Liaoning 114051,P.R.China)a(No.1 Senior High School of Anshan,Anshan,Liaoning 114051,P.R.China) |
| |
| Abstract: | Resveratrol was extracted from polygonum cuspidatum using ethanol as extraction solvent and determined by HPLC.The optimum detection conditions were：extend RP-ODS column 0.46cm×15cm,UV detector,240nm as detection wavelength,V（methanol）∶V（water）=50∶50 as mobile phase,elution flow rate of 1mL/min,room temperature.The method is sensitive,convenient,reliable and fast for on line detection of resveratrol and purification of resveratrol from polygonum cuspidatum. |
| |
| Keywords: | Polygonum Cuspidatum Resveratrol HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
