A potentiometric microbial sensor based on immobilized escherichia coli for glutamic acid |
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Authors: | Motohiko Hikuma Haruo Obana Takeo Yasuda Isao Karube Shuichi Suzuki |
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Institution: | Central Research Laboratories, Ajinomoto Co. Inc., 1 Suzuki-cho, Kawasaki-ku, Kawasaki 210 Japan;Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 227 Japan |
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Abstract: | The sensor consists of immobilized E. coli (which contains glutamate decarboxylase) and a carbon dioxide gas-sensor. Continuous introduction of sample solution into a flow system incorporating the sensor gives a potential which increases until a steady state is reached after 5 min. Measurements can also be made with only a 1- or 3-min introduction period with little loss of sensitivity. Calibration plots of mV measurements vs. logarithmic glutamic acid concentration are linear in the range 100–800 mg l-1. The sensor is highly selective, stable and reproducible. It has been applied to the determination of glutamic acid in fermentation broths. |
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