微流控芯片系统在单细胞研究中的应用

微流控芯片具有网络式通道结构,扩展了在细胞和亚细胞水平进行生命科学研究的能力,为单细胞研究提供了一个新的平台.在微流控芯片通道中,人们利用气压、液压和电压,或利用介电电泳、光学陷阱、行波介电电泳以及磁场等技术,可以操纵细胞通过或驻留在通道内的任意位置,从而使单细胞计数、筛选以及胞内组分分析等操作大大简化.本文对微流控芯片系统在血液流变学、单细胞操纵与计数以及单细胞胞内组分分析中的应用进行了综述,介绍了用于单细胞研究的多种微芯片系统,讨论了芯片上进行单细胞操纵的各种方法

Application of Microfluidic Chip Systems for the Research of Single Cell

The development of microfluidic chip system provides a new platform for the research of single cell. Because the sophistication and variety of microdevices and systems being built, the ability to study life at the cellular level and below is extended. On the microfluidic chip, an individual cell can be controlled and handled easily by use of pneumatic pressure, hydraulic pressure, voltage, dielectrophoresis force (DEP), optical trapper, traveling-wave dielectrophoresis (twDEP) and magnetic field, etc. The couplings of above techniques with microfluidic chip systems can operate the single cells to transit or dock anywhere in the micro channels. So the operations of single cell's counter, sorting and analysis were achieved conveniently. In this paper, the applications of microfliudic systems on blood rheology, counter or sorting, and intracellular contents analysis are reviewed. Several microfliudic chip systems used for study single cell are introduced. The operation methods on single cell are discussed.