Selection of carrier for immobilization of fructosyltransferase from Aureobasidium pullulans |
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Authors: | Z Platková M Polakovič V Štefuca M Vandáková M Antošová |
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Institution: | (1) Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia |
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Abstract: | The study deals with the development of an immobilized biocatalyst for the production of fructooligosaccharides. Several commercial
anion-exchange resins and polymethacrylate carriers were tested for the immobilization either by the direct attachment of
fructosyltransferase or by the attachment accompanied by simultaneous glutaraldehyde crosslinking in the pH range from 5.7
to 7.1. On the basis of the efficiency of immobilization and the storage stability, Amberlite IRA 900 and Dowex Marathon MSA
were selected as the best carriers for fructosyltransferase immobilization by direct attachment at pH 5.7.
Regular paper
An erratum to this article is available at . |
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Keywords: | fructosyltransferase immobilization Aureobasidium pullulans anion-exchange resins polymethacrylate carriers glutaraldehyde crosslinking |
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