| 黄瓜子叶培养物的花芽分化和ABP基因表达间的关系 |
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| 引用本文： | 杜勤,姜维梅,梁海曼. 黄瓜子叶培养物的花芽分化和ABP基因表达间的关系[J]. 浙江大学学报(理学版), 2000, 34(4): 459-463 |
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| 作者姓名： | 杜勤 姜维梅 梁海曼 |
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| 作者单位： | 浙江大学生命科学学院!浙江杭州310012 |
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| 基金项目： | 浙江省自然科学基金!(3980 82 ) |
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| 摘 要： | 黄瓜子叶培养物在ｐＨ７．０的培养基上培养１ｄ而后转入正常培养基，能显著提高黄瓜子叶培养物的成花率。因此ｐＨ７．０处理对黄瓜子叶柄同时具有促进ＡＢＰ基因表达和降低游离ＩＡＡ含量水平的作用。
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| 关 键 词： | IAA ABP 花芽分化 黄瓜 子叶培养物 基因表达 |
| 修稿时间： | 2000-03-26 |
The relationship of floral differentiation and ABP gene expression on cucumber cotyledon cultures. |
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| DU Qin,JIANG Wei|mei,LIANG Hai|man,ZHONG Hua|xin. The relationship of floral differentiation and ABP gene expression on cucumber cotyledon cultures.[J]. Journal of Zhejiang University(Sciences Edition), 2000, 34(4): 459-463 |
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| Authors: | DU Qin JIANG Wei|mei LIANG Hai|man ZHONG Hua|xin |
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| Abstract: | The frequency of floral differentiation improves highly(from 25% to 70%) when the detached cucumber cotyledons precultures with pH7.0 medium for 1 day. The endogenous levels of IAA in cotyledonary petiole where flower buds could appear were analysed. The results show that: the levels of IAA in cotyledonary petiole reduces with pH7.0 treated in contrast to the control(pH5.8). ABP gene expression were analysed in time 0-11 days of cotyledonary petiole,the results suggest that:its expression in all days is little strong with pH7.0 treated as compared with the control. So pH7.0 treatment not only promots the ABP gene expression but also reduces the endogenous levels of IAA in cotyledonary petiole. |
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| Keywords: | auxin ABP floral differentiation |
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