微波消解-电感耦合等离子体质谱法测定血液中30种微量元素

建立了微波消解-电感耦合等离子体质谱法定量测定血液中微量元素的方法.采用微波消解进行样品前处理,在硝酸-双氧水混合体系中消解后稀释,电感耦合等离子体质谱法(ICP-MS)测定全血中铜(Cu)、铅(Pb)、锌(Zn)、银(Ag)、钡(Ba)、锡(Sn)、镍(Ni)、钴(Co)、钒(V)、锑(Sb)、铊(Tl)、汞(Hg)、锰(Mn)、钼(Mo)、硒(Se)、铬(Cr)、镉(Cd)、钍(Th)、钛(Ti)、铝(Al)、砷(As)、铁(Fe)、钙(Ca)、镓(Ga)、锶(Sr)、锂(Li)、镁(Mg)、铍(Be)、钠(Na)和钾(K)元素的含量.对样品前处理过程和仪器工作参数进行了优化,通过内标校正法降低基体效应.结果表明,上述元素相关系数R^(2)均大于0.9998,检测精密度为0.142%~12.856%.方法简单、准确、快速,可精确测定全血中多种微量元素的含量,为全血中元素含量的测定提供了高效可靠的分析手段.

Determination of 30 Trace Elements in Blood by Microwave Digestion-Inductively Coupled Plasma-Mass Spectrometry

The method of determining 30 trace elements in blood has been developed using the microwave digestion-inductively coupled plasma-mass spectrometry. The samples were pretreated with a nitric acid-hydrogen peroxide mixed system with microwave digestion, and then diluted to be determined by inductively coupled plasma-mass spectrometry (ICP-MS). The contents of copper (Cu), lead (Pb), zinc (Zn), silver (Ag), barium (Ba), tin (Sn), nickel (Ni), cobalt (Co), vanadium (V), antimony (Sb), thallium (Tl), mercury (Hg), manganese (Mn), molybdenum (Mo), selenium (Se), chromium (Cr), cadmium (Cd), thorium (Th), titanium (Ti), aluminum (Al), arsenic (As), iron (Fe), calcium (Ca), gallium (Ga), strontium (Sr), lithium (Li), magnesium (Mg), beryllium (Be), sodium (Na) and potassium (K) in the whole blood were determined. The sample pretreatment process and all the instrument parameters were optimized. The matrix interference effect was reduced by the internal standard correction method. The results indicated that all the determined elements were in a good linear relationship with the response abundance of mass spectrometry in the corresponding concentration range. The detection precision was 0.142%~12.856%. The method has the advantages of simple, accurate and rapid, and is suitable for the accurate determination of the contents of various trace elements in the whole blood.