Rapid determination of propyphenazone in plasma by high-performance liquid chromatography. |
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Authors: | M C Rouan J Campestrini J B Lecaillon J Godbillon |
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Institution: | Laboratories CIBA-GEIGY, Biopharmaceutical Research Center, Rueil-Malmaison, France. |
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Abstract: | A rapid and simple high-performance liquid chromatographic assay for the determination of propyphenazone in plasma is described. Phenylbutazone was used as the internal standard. Plasma proteins were precipitated with acetonitrile before injection onto a 3-microns Supelcosil LC-18 column. The mobile phase, ethanol containing 0.2% (v/v) heptylamine-0.005 M potassium dihydrogenphosphate (30:70, v/v), was used at a flow-rate of 1.3 ml/min. The quantitation was performed by ultraviolet detection at a wavelength of 270 nm. The chromatographic time was 7 min. The within- and between-day coefficients of variation were less than 6% and the recoveries close to 100% for concentrations between 0.4 and 22 mumol/l. The limit of quantitation was 0.4 mumol/l (ca. 100 ng/ml). |
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