大豆多糖对尿石矿物草酸钙形成的调控作用

采用水提法从大豆中提取了大豆多糖(SPS),其多糖百分含量为97.38%;平均相对分子质量为115,200,羧基百分含量为13.8%。采用X射线衍射法(XRD)、红外光谱(FTIR)、扫描电子显微镜(SEM)和电感耦合等离子体发射光谱仪(ICP)研究了水溶性大豆多糖(SPS)对草酸钙晶体生长的调控作用。SPS大豆多糖不但可以抑制一水草酸钙(COM)晶体的聚集,而且可以诱导二水草酸钙(COD)晶体形成。当SPS浓度由0增大到0.50g·L-1时,草酸钙晶体由COM完全转变为COD晶体;并且随着多糖浓度的增大,溶解在溶液中的钙离子(可溶性钙)浓度显著增加,草酸钙沉淀的物质的量逐渐减少。这些结果表明,SPS有望成为预防草酸钙结石的一种绿色药物。

Modulation of Soluble Soybean Polysaccharide on Formation of Urinary Crystal Calcium Oxalate

The soluble soybean polysaccharide (SPS) was extracted from soybean by the method of water extraction. The content of polysaccharide in SPS was 97.38%; the average molecular weight of SPS was 115, 200, while the proportion of carboxyl groups (-COOH) in the molecular chains of SPS was 13.8%. Modifications of morphology and crystal phase of calcium oxalate were attempted via in vitro biomineralization method in soluble soybean polysaccharide (SPS) solution by means of X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy and ICP. SPS not only prevented calcium oxalate monohydrate (COM) crystals from aggregation, but also induced formation of thermodynamic metastable calcium oxalate dihydrate (COD) crystals. As the mass concentration of SPS increased from 0 to 0.50 g·L^-1, the percentage of COD increased from 0 to 100%, the soluble calcium ions increased rapidly, and the mole of precipitation of calcium oxalate crystals decreased. The results in this work showed that SPS may be a potential green drug for prevention of calcium oxalate urinary stones.