Thin-layer chromatography of radioactively labelled cholesterol and precursors from biological material

Summary The investigation methods of the action of xenobiotics on sterol biosynthesis from ¹⁴C-acetate in rat hepatocyte cultures can be developed, with regard to extraction using Extrelut and the separation of the sterol pattern by thin-layer chromatography, in such a way that they are suitable for wider application, e.g., screening. Good visualisation and recognition of changes in the sterol pattern are possible using autoradiography of the thin-layer chromatogram.

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Dünnschicht-Chromatographie von radioaktiv markiertem Cholesterin und Vorstufen aus biologischem Material. Eine einfache und empfindliche Methode zur Untersuchung einer Beeinflussung des Sterolbiosynthesewegs

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Dedicated to Dr. E. Roesch on occasion of his 65th birthday