Thin-layer chromatography of radioactively labelled cholesterol and precursors from biological material |
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| Authors: | J Pill J Aufenanger K Stegmeier F H Schmidt and D Müller |
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| Institution: | (1) Departments of Medical and Chemical Research, Boehringer Mannheim GmbH, Sandhofer Strasse 116, D-6800 Mannheim, Federal Republic of Germany |
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| Abstract: | Summary The investigation methods of the action of xenobiotics on sterol biosynthesis from 14C-acetate in rat hepatocyte cultures can be developed, with regard to extraction using Extrelut and the separation of the sterol pattern by thin-layer chromatography, in such a way that they are suitable for wider application, e.g., screening. Good visualisation and recognition of changes in the sterol pattern are possible using autoradiography of the thin-layer chromatogram.
Dünnschicht-Chromatographie von radioaktiv markiertem Cholesterin und Vorstufen aus biologischem Material. Eine einfache und empfindliche Methode zur Untersuchung einer Beeinflussung des Sterolbiosynthesewegs
Dedicated to Dr. E. Roesch on occasion of his 65th birthday |
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