Fluorescent probes for in vitro and in vivo quantification of hydrogen peroxide |
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| Authors: | Sen Ye Jun Jacob Hu Qian Angela Zhao Dan Yang |
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| Institution: | Department of Chemistry, Morningside Laboratory for Chemical Biology, The University of Hong Kong Shenzhen Institute of Research and Innovation (HKU-SIRI), The University of Hong Kong, Pokfulam Road, Hong Kong P. R. China, |
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| Abstract: | Hydrogen peroxide (H2O2) plays essential roles in redox signaling and oxidative stress, and its dynamic concentration is critical to human health and diseases. Here we report the design, syntheses, and biological applications of HKPerox-Red and HKPerox-Ratio for quantitative measurement of H2O2. Both probes were successfully applied to detect endogenous H2O2 fluxes in living cells or zebrafish, and biological effects of multiple stress inducers including rotenone, arsenic trioxide, and starvation were investigated. As H2O2 is a common by-product for oxidase oxidation, a general assay was developed for ultrasensitive detection of various metabolites (glucose, uric acid, and sarcosine). Moreover, cellular H2O2 measurements were achieved for the first time by combining flow cytometry with live cell calibration. This study provides a pair of unique molecular tools for advanced H2O2 bio-imaging and assay development. New class of H2O2 probes, HKPerox-Red and HKPerox-Ratio, were developed for quantitative measurement of H2O2 generated in multiple disease models using bio-imaging, flow cytometry, and in vitro assays in an ultra-sensitive and selective manner. |
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