Complexing DNA Origami Frameworks through Sequential Self‐Assembly Based on Directed Docking |
| |
| Authors: | Dr. Yuki Suzuki Prof. Dr. Hiroshi Sugiyama Prof. Dr. Masayuki Endo |
| |
| Affiliation: | 1. Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan;2. Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan;3. Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, Japan |
| |
| Abstract: | Ordered DNA origami arrays have the potential to compartmentalize space into distinct periodic domains that can incorporate a variety of nanoscale objects. Herein, we used the cavities of a preassembled 2D DNA origami framework to incorporate square‐shaped DNA origami structures (SQ‐origamis). The framework was self‐assembled on a lipid bilayer membrane from cross‐shaped DNA origami structures (CR‐origamis) and subsequently exposed to the SQ‐origamis. High‐speed AFM revealed the dynamic adsorption/desorption behavior of the SQ‐origamis, which resulted in continuous changing of their arrangements in the framework. These dynamic SQ‐origamis were trapped in the cavities by increasing the Mg2+ concentration or by introducing sticky‐ended cohesions between extended staples, both from the SQ‐ and CR‐origamis, which enabled the directed docking of the SQ‐origamis. Our study offers a platform to create supramolecular structures or systems consisting of multiple DNA origami components. |
| |
| Keywords: | directed assembly DNA origami high-speed atomic force microscopy lipid bilayers self-assembly |
|
|
