| 微胶囊固定化过氧化氢酶 |
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| 引用本文： | 王玉洁,刘持标.微胶囊固定化过氧化氢酶[J].高等学校化学学报,1996,17(6):953-956. |
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| 作者姓名： | 王玉洁 刘持标 |
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| 作者单位： | 东北师范大学化学系，中国科学院长春应用化学研究所 |
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| 基金项目： | 国家自然科学基金重点基金 |
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| 摘 要： | 以乙基纤维素为膜材，用液中干燥法将过氧化氢酶微胶囊化，研究了温度和ｐＨ值对酶活性的影响及过氧化氢和尿素对固定化酶的抑制作用，由于乙基纤维素膜的保护，明胶对ｐＨ值的缓冲作用及明胶与尿素的反应，明显地扩大了酶对温度和ｐＨ值的适应性，降低了酶活性抑制剂的影响。
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| 关 键 词： | 微胶囊 过氧化氢酶 固定化酶 酶活性 |
Immobilized Catalase with Microcapsules |
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| WANG Yu-Jie,LIU Chin-Biao, BAI Guang-Yue,CHU Ying,WANG Feng-Ping MA Zhan-Fang,ZHANG He-Zhe.Immobilized Catalase with Microcapsules[J].Chemical Research In Chinese Universities,1996,17(6):953-956. |
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| Authors: | WANG Yu-Jie LIU Chin-Biao BAI Guang-Yue CHU Ying WANG Feng-Ping MA Zhan-Fang ZHANG He-Zhe |
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| Abstract: | Catalase was microencapsulated with ethyl cellulose as a film material by a method of drying in liquid.It was investigated that the immobilized catalase was affected by temperature and pH and inhibited by hydrogen peroxide.The available temperature and pH ranges of the microencapsulated catalase owing to the protection of ethyl cellulose film and the buffer action of gelatine and phosphoric acid separately,and the inhibition, because of the presence of gelatine,are enlarged clearly. |
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| Keywords: | Microcapsule Catalase Immobilized enzyme |
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