明胶固定辣根过氧化物酶制备H_2O_2传感器

用明胶将辣根过氧化物酶(HRP)固定于多壁碳纳米管(MWNT)和茜素红(AR)修饰的玻碳(GC)电极上,制成HRP生物传感器(HRP/AR/MWNT/GC),然后在3%戊二醛(GA)中进行交联改性,以克服明胶膜易溶胀的缺点,并提高膜的稳定性.同时详细探讨了该传感器对H2O2的响应性能,并优化了实验条件.结果表明,该传感器对H2O2的线性响应范围为5.0×10-6～1.0×10-3mol/L,线性相关系数为0.9932,检出限为1.0×10-7mol/L,且放于4℃环境30d后,峰电流值约为原来的72.1%.该传感器响应快速,灵敏度高,且具有良好的重现性、稳定性及较长的使用寿命,具有潜在的应用价值.

Hydrogen Peroxide Biosensor Based on Gelatin Immobilizing Horseradish Peroxidase

A novel hydrogen peroxide biosensor was obtained by successfully immobilizing horseradish peroxidase(HRP) on a glassy carbon(GC) electrode,which was modified with multi-walled carbon nanotubes(MWNT) and alizarin red(AR).Then the biosensor was immerged in 3% glutaraldehyde(GA) in order to overcome the swelling and improve the stability of the gelatin membrane.The experimental condition was optimized,and the electrochemistry behavior of the HRP/AR/MWNT/GC biosensor in PBS(pH 7.0) and H2O2 was discussed.The bi...