Sensor System for Simultaneous Measurement of Oxygen and Hydrogen Peroxide Concentration During Glucose Oxidase Activity |
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| Authors: | P Westbroek E Temmerman F Govaert P Kiekens J De Strycker |
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| Institution: | 1. University of Gent, Department of Analytical Chemistry, Krijgslaan 281 S12, B-9000 Gent, Belgium;2. University of Gent, Department of Textiles, Technologiepark 9, B-9052 Zwijnaarde, Belgium |
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| Abstract: | An amperometric sensor system, based on a repetitive double step potential method at a glassy carbon electrode, has been developed for the simultaneous measurement of hydrogen peroxide and oxygen concentrations. The current measured at a potential of –1 V (vs. Ag/AgCl/saturated Cl–) corresponds to the sum of the reduction currents of hydrogen peroxide and dissolved oxygen. The current measured at –0.55 V (vs. Ag/AgCl/saturated Cl–) is due to the reduction of dissolved oxygen to hydrogen peroxide. Alternatively, the concentration of dissolved oxygen can also be determined using a Clark electrode. The concentration of hydrogen peroxide and dissolved oxygen during enzymatic conversion of glucose can be followed on line and be used to control the process. |
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| Keywords: | Simultaneous measurements Hydrogen peroxide Oxygen Glucose oxidase Repetitive double step potential method |
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