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Production of recombinant bleaching enzymes from thermophilic microorganisms in fungal hosts |
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| Authors: | Bergquist Peter L. Te’o V. S. Junior Gibbs Moreland D. Cziferszky Angela C. E. De Faria Fabricia P. Azevedo Maristela O. Nevalainen K. M. Helena |
| Affiliation: | (1) Department of Biological Sciences, Macquarie University, 2109 Sydney, NSW, Australia;(2) Division of Molecular Medicine, University of Auckland Medical School, 1000 Auckland, New Zealand;(3) Institute of Biochemical Technology and Microbiology, Section of Microbial Biochemistry, Technical University of Vienna, A-1040 Vienna, Austria;(4) Department de Biotecnologia, FAENQUIL, CP116, Lorena-SP, Brazil;(5) Department de Biologia Celular, IB UnB 70.910-900, Brasilia-DF, Brazil |
| Abstract: | Cost-effective production of enzymes for industrial processes makes the appropriate selection of the host-vector expression system critical. We have developed two systems for the bulk production of bleaching enzymes from thermophiles. Kluyveromyces lactis has been developed as a secretion host employing expression vectors based on the 2μ-like plasmid pKD1 of Kluyveromyces drosophilarium. Our second system involves the filamentous fungus Trichoderma reesei. Fusion and nonfusion vectors have been constructed using the strong cellobiohydrolase 1 (cbh1) promoter. The KEX2 protease cleavage site and a 6 × HIS-tag have been incorporated to facilitate both cleavage and purification of the mature foreign proteins. |
| Keywords: | Trichoderma reesei Kluyveromyces lactis thermophilic xylanases heterologous expression gene redesign |
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