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Novel functional activities of anti-dna autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases |
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| Authors: | A V Kozyr A V Kolesnikov E S Aleksandrova L P Sashchenko N V Gnuchev P V Favorov M A Kotelnikov E I Iakhnina I A Astsaturov T B Prokaeva Z S Alekberova S V Suchkov A G Gabibov |
| Institution: | (1) Shemyokin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St., 16/10, 117871, V-437 Moscow, Russia;(2) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia;(3) Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia;(4) Russian Hematological Center, Russian Academy of Medical Sciences, Moscow, Russia;(5) Institute of Rheumatology, Russian Academy of Medical Sciences, Moscow, Russia;(6) Moscow Regional Research Institute of Clinical Investigations, Moscow, Russia |
| Abstract: | DNA-hydrolyzing activity of IgG autoantibodies from sera of patients with various types of lymphoproliferative diseases was investigated. The association of DNA-hydrolyzing activity with the antibody (Ab) fraction has been proved by newly developed affinity-capture assay. Study of abzyme incidence in blood tumors and systemic lupus erythematosis (SLE) revealed linkage of anti-DNA Ab catalysts to mature B-cell tumors, and increased probability of DNA-abzymes formation on the background of autoimmune manifestations. These data suggest possible similarity between mechanisms of abzyme formation in SLE and B-cell lymphomas. A new mechanism of formation of DNA-specific catalytic Abs has been proposed based on the increased crossreactivity of polyclonal DNA-abzymes to DNA-depleted nuclear matrix proteins. The possibility of the abzyme production as Ab to the energetically destabilized ground state of the antigen has been discussed. Preliminary results were obtained that indicate the complement-independent cytotoxicity of anti-DNA autoantibodies isolated from blood of patients with SLE and chronic lymphocytic leukemia. |
| Keywords: | DNA-hydrolyzing antibodies lymphoproliferative diseases chronic lymphocytic leukemia affinity-capture assay autoantibody cytotoxicity DNA-protein complexes nuclear matrix |
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