d(C4)相关序列形成四分子i-Motif结构的电喷雾质谱研究

设计了与富含胞嘧啶(C)的DNA序列d(C4)相关的DNA序列d(C4),d(TC4),d(AC4),d(T2C4),d(A2C4),d(C4T),d(C4A)和d(TC4T)；采用电喷雾质谱测定发现这些序列形成四分子非共价复合物离子,根据离子的相对丰度可确定形成四链i-motif结构的数量和可能性；同时考察了腺嘌呤(...

Electrospray Ionization Mass Spectrometry Studies of Tetramolecular i-Motif Structures Formed by d(C4) Related Sequences

A series of cytosine-rich DNA sequence d(C4) related DNA strands, including d(C4), d(TC4), d(AC4), d(T2C4), d(A2C4), d(C4T), d(C4A) and d(TC4T), was designed in this study, and the tetramolecular ions formed by the sequences were measured with electrospray ionization mass spectrometry. The goal is to obtain the information on the formation of the tetramolecular i-motif influenced by adenine(A) and thymine(T) that present at 5' and 3' ends of d(C4). The results showed that when A base or T base was added at 5' end of d(C4), it is easier for the sequence to form tetramolecular ion, especially for thymine, when adding two A or T bases to d(C4), the sequence generates higher abundant of the tetramolecualr ion.