Phytase Production by a Marine Yeast <Emphasis Type="Italic">Kodamea ohmeri</Emphasis> BG3 |
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Authors: | Xiaoyu Li Zhenming Chi Zhiqiang Liu Kuirang Yan Huijuan Li |
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Institution: | (1) Unesco Chinese Center of Marine Bhitechnology, Ocean University of China, Yushan Road, no. 5, Qingdao, China |
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Abstract: | The marine yeast strain Kodamea ohmeri BG3 isolated from the gut of a marine fish (Hexagrammes otakii) was found to secrete a large amount of phytase into the medium. The crude phytase produced by this marine yeast showed the
highest activity at pH 5.0 and 65 °C. The optimal medium for phytase production contained oat 10.0 g/l, ammonium sulfate 15.0 g/l,
glucose 30 g/l, and NaCl 20.0 g/l, while the optimal cultivation conditions for phytase production were pH 5.0, a temperature
of 28 °C, and a shaking speed of 170 rpm. Under the optimal conditions, over 557.9 mU/ml of phytase activity was produced
within 72 h of fermentation at the shake flask level. This is a very high level of phytase activity produced by yeasts. We
think that the medium and process for phytase production by the marine yeast strain were very simple, and such marine yeast
from the gut of natural marine fish may have a potential application in the maricultural industry and marine environmental
protection. The results demonstrate that phytate was actively degraded by the crude phytase within a short period. |
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Keywords: | Marine yeasts Phytase Kodamea ohmeri Marine fish Oat |
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