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Determination of phenylalanine in blood by high-performance anion-exchange chromatography–pulsed amperometric detection to diagnose phenylketonuria |
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| Authors: | Ji-Seon Jeong Hee-Jung Sim Yong-Moon Lee Hye-Ran Yoon Dong Hwan Lee Seon-Pyo Hong |
| Institution: | 1. Department of Oriental pharmaceutical sciences and Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, 1 Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea;2. College of Pharmacy, CBITRC, Chungbuk National University, Chongju 361-763, South Korea;3. Biomedical and Pharmaceutical Analysis Laboratory, College of Pharmacy, Duksung Women''s University, 419 Ssangmoon-dong, Dobong-gu, Seoul 132-714, South Korea;4. Department of Pediatrics, Soonchunhyang University Hospital, 657 Hannam-dong, Yongsan-gu, Seoul 140-743, South Korea |
| Abstract: | We have developed a high-performance anion-exchange chromatography with pulsed amperometric detection method for the detection of phenylalanine (Phe) and diagnosis of phenylketonuria (PKU). Sample pretreatment steps were simplified without derivatization. The analyte was separated within 5 min. The detection limit (S/N = 3) for Phe was 50 pg. Linear dynamic range was 1.23–14.43 mg/dL (r2 = 0.9999) for a dried blood spot. The mean recoveries of Phe for intra- and inter-day assays were found to be 96.87–104.16%. This method clearly differentiated PKU-positive groups from normal groups, and proved to be a practical procedure for rapid screening and follow-up monitoring of PKU. |
| Keywords: | Phenylketonuria Dried blood spot Phenylalanine Rapidity High throughput Pulsed amperometric detection |
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