Quantitation of carcinogen bound protein adducts by fluorescence measurements |
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| Affiliation: | 2. Berthiaume Institute for Precision Health, University of Notre Dame, Notre Dame, Indiana;3. Mike and Josie Harper Cancer Research Institute, University of Notre Dame, Notre Dame, Indiana;4. Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana |
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| Abstract: | ![]()
A highly significant correlation of aflatoxin B1 serum albumin adduct level with daily aflatoxin B1 intake was observed in a molecular epidemiological study of aflatoxin carcinogenesis which used conventional fluorescence spectroscopy methods for adduct quantitation. Synchronous fluorescence spectroscopy and laser induced fluorescence techniques have been employed to quantitate antibenzo[a]pyrene diol epoxide derived globin peptide adducts. Fast and efficient methods to isolate the peptide adducts as well as eliminate protein fluorescence background are described. A detection limit of several femtomoles has been achieved. Experimental and technical considerations of low temperature synchronous fluorescence spectroscopy and fluorescence line narrowing to improve the detection sensitivities are also presented. |
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