Directed Evolution of Human Cu,Zn-SOD Under Artificial Oxidant Pressures |
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Authors: | Gou Xiao-jun LI Chun-lei KONG Xiang-duo ZHU Shi-zhen ZHANG Hong-ying WANG Xiao-ping ZHANG Jin Liu Tian-yun |
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Institution: | 1. Key Lab for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, P. R. China;
2. The People's Hospital of Tongjiang Country, Tongjiang 635700, P. R. China |
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Abstract: | Cu,Zn SOD is a highly conserved enzyme and the controversy about its evolutionary possibility in the near future has been lively. In order to further our understanding of the future fate of human Cu,Zn SOD, we adopted a strategy relating to the directed evolution to study how the mutants of human Cu,Zn SOD respond to different oxidative stress. After five rounds of screening, we found a mutant that can survive under harsh pressures and DNA sequencing proves that it shows a mutation responsible for the phenomenon. However, under natural pressure, our screening comes to nothing. Then we may draw the following conclusions: the evolution of biological macromolecules in some respect depends on their surroundings and if they are too familiar with a certain environment, they may embody evolutionary inertia. |
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Keywords: | Human Cu Zn SOD Directed evolution Screening |
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