超高效液相色谱法结合化学计量学分析评价4种商品人参的质量

建立了快速、灵敏、准确的超高效液相色谱方法,用来分析4种商品人参(人参、红参、人参叶、人参须)中12种人参皂苷的含量,并用化学计量学方法评价了商品人参的质量。采用ACQUITY UPLC^TM BEH C₁₈色谱柱(50 mm×2.1 mm, 1.7 μm),以乙腈-水为流动相进行梯度洗脱。对所建立的测定12种人参皂苷的UPLC方法进行了线性方程、准确度、重复性、回收率等方法学考察。采用聚类分析和主成分分析的化学计量学方法对4种商品人参进行分析,评价了其质量。结果表明聚类分析和主成分分析2种化学计量学方法非常适合大样本、多成分的中药材质量分析。

Analysis and assessment of four commercial products of Asian ginseng by ultra-performance liquid chromatography and chemometric analysis

Ginseng Radix et Rhizoma (GRR, also named as white ginseng), Ginseng Radix et Rhizoma Rubra (GRR Rubra, also named as red ginseng), Ginseng Folium (GF) and Ginseng Rootlet (GR) products from Asian ginseng, one of the well-known Chinese traditional medicine for thousands of years, are now widely used around the world. Thus the comprehensive quality control is of paramount concern basing on the contents of the bioactive ginsenosides. A rapid, sensitive and reliable method of ultra-performance liquid chromatography coupled with a photodiode array detection (UPLC-PAD) was developed for the quantitative analysis of the 12 ginsenosides in the four commercial ginseng products of Asian ginseng. The chromatography was performed on an ACQUITY UPLC^TM BEH C₁₈ column using a gradient elution with acetonitrile/water as the mobile phases. Method validation including calibration curves, accuracies, precisions, repeatabilities and recoveries was investigated. The contents of the 12 ginsenosides were determined in 20 GRR, 4 GF, 4 GR and 11 GRR Rubra samples. To evaluate the sample quality, chemometric methods including hierarchical cluster analysis (HCA) and principal components analysis (PCA) were engaged in evaluating the GRR, GRR Rubra, GF and GR products from Asian ginseng. The results showed that HCA and PCA can be considered as the attractive chemometric techniques in situations where high sample throughput and multiple analytes are required.