| 生物素标识酶在生物传感器中的应用研究 |
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| 引用本文： | 卢昕,何品刚,方禹之.生物素标识酶在生物传感器中的应用研究[J].高等学校化学学报,1995,16(11):1690-1693. |
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| 作者姓名： | 卢昕 何品刚 方禹之 |
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| 作者单位： | 华东师范大学化学系, 上海, 200062 |
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| 基金项目： | 国家教育委员会科研基金 |
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| 摘 要： | 利用生物素与亲合素之间的特异性结合,在恒电位下,首先将亲合素吸附在电极上,然后使标识在生物素上的葡萄糖氧化酶固定于电极表面,制成具有高活性的多层固定化酶的葡萄糖传感器。电极线性范围:1.0×10-5～1.0×10-2mol/L,检测限:6.0×10-6mol/L,响应时间:6s.本传感器具有线性范围宽、灵敏度高、响应速度快、可反复活化使用等优点。
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| 关 键 词： | 亲合素 生物素标识葡萄糖氧化酶 葡萄糖传感器 |
| 收稿时间： | 1994-12-21 |
The Application of Biotinylated Enzyme in Biosensor |
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| LU Xin, HE Pin-Gang, FANG Yu-Zhi.The Application of Biotinylated Enzyme in Biosensor[J].Chemical Research In Chinese Universities,1995,16(11):1690-1693. |
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| Authors: | LU Xin HE Pin-Gang FANG Yu-Zhi |
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| Institution: | Department of Chemistry, East China Normal Univcrsity, Shanghai, 200062 |
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| Abstract: | This paper describes the preparation of enzyme electrode with active multilayers glucose oxidase utilizing avidin-biotin technique under +0.6V potential, avidin is adsorbed on Pt electrode,then active multilayers of glucose oxidase can be immobillized with avidin bi-otinylated glucose oxidase complex. The biosensor has the characteristies of high sensitivity,wide response range,fast responding,and it can be repeatedly activized with avidin biotinylated enzyme complex solution. The limit of detection for glucose is 6. 0× 10-6mol/L. The linear relationship between △iand concentration of glucose holds in the range of 1.0× 10-5～1.0× 10-2mol/L. |
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| Keywords: | Avidin Biotinglated enzyme Biosensor |
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