Metabolic profiling of urinary organic acids by single and multicolumn capillary gas chromatography |
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| Authors: | M F Lefevere B J Verhaeghe D H Declerck J F Van Bocxlaer A P De Leenheer R M De Sagher |
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| Affiliation: | Laboratoria voor Medische Biochemie en voor Klinische Analyse, Gent, Belgium. |
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| Abstract: | ![]()
High-resolution gas chromatography (HRGC) and gas chromatography/mass spectrometry (GC/MS) are the techniques of choice to determine the retention indices of more than 200 organic acids as their trimethylsilyl (TMS) or oxime-trimethylsilyl derivatives. Several types of apolar and semipolar fused-silica capillary columns (OV-1, SE-52, and OV-1701), used to analyze and separate organic acids isolated from urine samples, are evaluated. |
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