超高效液相色谱-串联四极杆飞行时间质谱法快速分析东革阿里化学成分

应用超高效液相色谱-串联四极杆飞行时间质谱法(UPLC-Q-TOF-MS)对东革阿里提取物的化学成分进行定性分析。采用Agilent Eclipse Plus-C18 RRHD色谱柱(2.1 mm×100 mm,1.8μm),以乙腈-0.1%甲酸水为流动相进行梯度洗脱。质谱分析采用电喷雾正离子模式采集,通过对照品比对及高分辨质谱数据解析,鉴定了49种化学成分,包括29种苦木素二萜、4种β-咔巴啉类生物碱、12种铁屎米-6-酮类生物碱、3种联苯新木脂素类及1种角鲨烯型三萜。该方法快速、可靠、高效,能较全面地反映东革阿里的化学成分,可为深入阐明东革阿里的药效物质基础及质量标准研究提供参考。

Rapid Analysis of Chemical Constituents in Eurycoma longifolia Jack by Ultra performance Liquid Chromatography-Quadrupole Time of flight Mass Spectrometry

An ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometric(UPLC-Q-TOF-MS)method was established for the identification of chemical constituents in the extract of Eurycoma longifolia Jack.The sample was separated on an Agilent Eclipse Plus-C18 RRHD(2.1 mm×100 mm,1.8μm)column with acetonitrile and 0.1%formic acid water as mobile phases by gradient elution.The data were collected with electrospray ionization in positive mode.According to the contrast of the reference standards and the accurate masses of molecules,a total of 49 compounds in Eurycoma longifolia Jack extract were identified,including 29 quassinoid diterpenoids,4β-carboline alkaloids,12 canthin-6-one alkaloids,3 biphenyl-neolignans and 1 squalene triterpene.The method was accurate,reliable and efficient,and could comprehensively reflect the chemical constituents of Eurycoma longifolia Jack,and provided a reference for further elucidating its pharmacological basis and quality controll.